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cloneexpress multis one step cloning kit  (Vazyme Biotech Co)


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    Structured Review

    Vazyme Biotech Co cloneexpress multis one step cloning kit
    Cloneexpress Multis One Step Cloning Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 97/100, based on 1456 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cloneexpress+multis+one+step+cloning+kit/ClonExpress+MultiS+One+Step+Cloning+Kit/pmc12809138-48-0-7
    Average 97 stars, based on 1456 article reviews
    cloneexpress multis one step cloning kit - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Cloning:

    Article Title: Protocol for studying in vitro mouse primary thymocyte differentiation via retroviral-mediated gene expression and stromal-free stimulation
    Article Snippet: MojoSort Streptavidin Nanobeads , BioLegend , Cat# 480016. .. cloneExpress MultiS One Step Cloning Kit , Vazyme , Cat# C113. .. TIANprep Midi Plasmid Kit , TianGen , Cat# DP106-02.

    Article Title: H + -ATPase subunit a3 (MdVHA-a3) orchestrates Proton Gradient-Driven Ion Homeostasis and Salt Tolerance via a Non-canonical SOS pathway in apple.
    Article Snippet: Soil salinization imposes catastrophic constraints on Malus domestica productivity, primarily through ionic toxicity and osmotic stress that disrupt cellular homeostasis.. Salt stress constitutes a primary environmental constraint limiting the geographic distribution, productivity, and yield security of Malus species.. Here, we characterize the vacuolar H+-ATPases (VHAs) subunit MdVHA-a3 as a master regulator of apple salt adaptation.

    Article Title: Glutamine signaling specifically activates c-Myc and Mcl-1 to facilitate cancer cell proliferation and survival
    Article Snippet: .. Whole length human FBW7, QARS, c-MYC, MCL-1, NOTCH1, CCNE1, and JUN were amplified from HCT116 +/+ cDNA and cloned into the Xho I and EcoR I restriction sites of the pcDNA3.1-Flag/HA/Myc vector using CloneExpress multiS One Step Cloning Kit (#C113-02, Vazyme, Nanjing, China). .. Site-directed mutagenesis of FBW7 was performed with the Mut Express MultiS Fast Mutagenesis kit (#C215-01, Vazyme).

    Article Title: Genome mining of nonenzymatic ortho-quinone methide-based pseudonatural products from ascidian-derived fungus Diaporthe sp.SYSU-MS4722
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Vitamin C inactivates c-Jun N-terminal kinase to stabilize heart and neural crest derivatives expressed 1 (Hand1) in regulating placentation and maintenance of pregnancy
    Article Snippet: .. Constitutive active forms of JNKs (JNK*), including Flag-JNKK2-JNK1 (JNK1*) and Flag-JNKK2-JNK2 (JNK2*) were constructed through fusing JNKK2 with JNK1 or JNK2 fragments (cDNA gifted from Professor Jiahuai Han at Xiamen University, Xiamen, China) together by CloneExpress MultiS One Step Cloning Kit (Vazyme, Nanjing, China) and specified primers (Supplementary Table 2). .. JEG-3 or 293T cells transfected with Hand1 variants, JNK1*/2*, HA-ubiquitin (gifted from Dr. Lihui Li at Fudan University, Shanghai, China) for 6~8 h by using Lipofectamine 3000 (Life Technologies).

    Article Title: Glutamine signaling specifically activates c-Myc and Mcl-1 to Facilitate cancer cell proliferation and survival.
    Article Snippet: .. Whole length human FBW7, QARS, c-MYC, MCL-1, NOTCH1, CCNE1 and JUN were amplified from HCT116 +/+ cDNA and cloned into the Xho I and EcoR I restriction sites of the pcDNA3.1-Flag/HA/Myc vector using CloneExpress multiS One Step Cloning Kit (#C11302, Vazyme, Nanjing, China). .. Site-directed mutagenesis of FBW7 was performed with the Mut Express MultiS Fast Mutagenesis kit (#C215-01, Vazyme).

    Article Title: Tryptophanylation of insulin receptor by WARS attenuates insulin signaling
    Article Snippet: .. Plasmids construction and transfection Whole length human WARS, IR, and SIRT1 were amplified HEK293T cDNA and cloned into Xho I and EcoR I sites of the pcDNA3.1-Flag/HA vector using CloneExpress MultiS One Step Cloning Kit (Vazyme, #C113-02, CHN). .. The mutants were generated by site-directed mutagenesis using the Mut Express MultiS Fast Mutagenesis Kit (Vazyme, #C215-01, CHN) according to the manufacturer’s instructions.

    Article Title: Tryptophanylation of insulin receptor by WARS attenuates insulin signaling
    Article Snippet: .. Whole length human WARS, IR, and SIRT1 were amplified HEK293T cDNA and cloned into Xho I and EcoR I sites of the pcDNA3.1-Flag/HA vector using CloneExpress MultiS One Step Cloning Kit (Vazyme, #C113-02, CHN). .. The mutants were generated by site-directed mutagenesis using the Mut Express MultiS Fast Mutagenesis Kit (Vazyme, #C215-01, CHN) according to the manufacturer’s instructions.

    Polymerase Chain Reaction:

    Article Title: H + -ATPase subunit a3 (MdVHA-a3) orchestrates Proton Gradient-Driven Ion Homeostasis and Salt Tolerance via a Non-canonical SOS pathway in apple.
    Article Snippet: Soil salinization imposes catastrophic constraints on Malus domestica productivity, primarily through ionic toxicity and osmotic stress that disrupt cellular homeostasis.. Salt stress constitutes a primary environmental constraint limiting the geographic distribution, productivity, and yield security of Malus species.. Here, we characterize the vacuolar H+-ATPases (VHAs) subunit MdVHA-a3 as a master regulator of apple salt adaptation.

    Plasmid Preparation:

    Article Title: H + -ATPase subunit a3 (MdVHA-a3) orchestrates Proton Gradient-Driven Ion Homeostasis and Salt Tolerance via a Non-canonical SOS pathway in apple.
    Article Snippet: Soil salinization imposes catastrophic constraints on Malus domestica productivity, primarily through ionic toxicity and osmotic stress that disrupt cellular homeostasis.. Salt stress constitutes a primary environmental constraint limiting the geographic distribution, productivity, and yield security of Malus species.. Here, we characterize the vacuolar H+-ATPases (VHAs) subunit MdVHA-a3 as a master regulator of apple salt adaptation.

    Homologous Recombination:

    Article Title: H + -ATPase subunit a3 (MdVHA-a3) orchestrates Proton Gradient-Driven Ion Homeostasis and Salt Tolerance via a Non-canonical SOS pathway in apple.
    Article Snippet: Soil salinization imposes catastrophic constraints on Malus domestica productivity, primarily through ionic toxicity and osmotic stress that disrupt cellular homeostasis.. Salt stress constitutes a primary environmental constraint limiting the geographic distribution, productivity, and yield security of Malus species.. Here, we characterize the vacuolar H+-ATPases (VHAs) subunit MdVHA-a3 as a master regulator of apple salt adaptation.

    Over Expression:

    Article Title: H + -ATPase subunit a3 (MdVHA-a3) orchestrates Proton Gradient-Driven Ion Homeostasis and Salt Tolerance via a Non-canonical SOS pathway in apple.
    Article Snippet: Soil salinization imposes catastrophic constraints on Malus domestica productivity, primarily through ionic toxicity and osmotic stress that disrupt cellular homeostasis.. Salt stress constitutes a primary environmental constraint limiting the geographic distribution, productivity, and yield security of Malus species.. Here, we characterize the vacuolar H+-ATPases (VHAs) subunit MdVHA-a3 as a master regulator of apple salt adaptation.

    Construct:

    Article Title: H + -ATPase subunit a3 (MdVHA-a3) orchestrates Proton Gradient-Driven Ion Homeostasis and Salt Tolerance via a Non-canonical SOS pathway in apple.
    Article Snippet: Soil salinization imposes catastrophic constraints on Malus domestica productivity, primarily through ionic toxicity and osmotic stress that disrupt cellular homeostasis.. Salt stress constitutes a primary environmental constraint limiting the geographic distribution, productivity, and yield security of Malus species.. Here, we characterize the vacuolar H+-ATPases (VHAs) subunit MdVHA-a3 as a master regulator of apple salt adaptation.

    Article Title: Vitamin C inactivates c-Jun N-terminal kinase to stabilize heart and neural crest derivatives expressed 1 (Hand1) in regulating placentation and maintenance of pregnancy
    Article Snippet: .. Constitutive active forms of JNKs (JNK*), including Flag-JNKK2-JNK1 (JNK1*) and Flag-JNKK2-JNK2 (JNK2*) were constructed through fusing JNKK2 with JNK1 or JNK2 fragments (cDNA gifted from Professor Jiahuai Han at Xiamen University, Xiamen, China) together by CloneExpress MultiS One Step Cloning Kit (Vazyme, Nanjing, China) and specified primers (Supplementary Table 2). .. JEG-3 or 293T cells transfected with Hand1 variants, JNK1*/2*, HA-ubiquitin (gifted from Dr. Lihui Li at Fudan University, Shanghai, China) for 6~8 h by using Lipofectamine 3000 (Life Technologies).

    Amplification:

    Article Title: Glutamine signaling specifically activates c-Myc and Mcl-1 to facilitate cancer cell proliferation and survival
    Article Snippet: .. Whole length human FBW7, QARS, c-MYC, MCL-1, NOTCH1, CCNE1, and JUN were amplified from HCT116 +/+ cDNA and cloned into the Xho I and EcoR I restriction sites of the pcDNA3.1-Flag/HA/Myc vector using CloneExpress multiS One Step Cloning Kit (#C113-02, Vazyme, Nanjing, China). .. Site-directed mutagenesis of FBW7 was performed with the Mut Express MultiS Fast Mutagenesis kit (#C215-01, Vazyme).

    Article Title: Glutamine signaling specifically activates c-Myc and Mcl-1 to Facilitate cancer cell proliferation and survival.
    Article Snippet: .. Whole length human FBW7, QARS, c-MYC, MCL-1, NOTCH1, CCNE1 and JUN were amplified from HCT116 +/+ cDNA and cloned into the Xho I and EcoR I restriction sites of the pcDNA3.1-Flag/HA/Myc vector using CloneExpress multiS One Step Cloning Kit (#C11302, Vazyme, Nanjing, China). .. Site-directed mutagenesis of FBW7 was performed with the Mut Express MultiS Fast Mutagenesis kit (#C215-01, Vazyme).

    Article Title: Tryptophanylation of insulin receptor by WARS attenuates insulin signaling
    Article Snippet: .. Plasmids construction and transfection Whole length human WARS, IR, and SIRT1 were amplified HEK293T cDNA and cloned into Xho I and EcoR I sites of the pcDNA3.1-Flag/HA vector using CloneExpress MultiS One Step Cloning Kit (Vazyme, #C113-02, CHN). .. The mutants were generated by site-directed mutagenesis using the Mut Express MultiS Fast Mutagenesis Kit (Vazyme, #C215-01, CHN) according to the manufacturer’s instructions.

    Article Title: Tryptophanylation of insulin receptor by WARS attenuates insulin signaling
    Article Snippet: .. Whole length human WARS, IR, and SIRT1 were amplified HEK293T cDNA and cloned into Xho I and EcoR I sites of the pcDNA3.1-Flag/HA vector using CloneExpress MultiS One Step Cloning Kit (Vazyme, #C113-02, CHN). .. The mutants were generated by site-directed mutagenesis using the Mut Express MultiS Fast Mutagenesis Kit (Vazyme, #C215-01, CHN) according to the manufacturer’s instructions.

    Clone Assay:

    Article Title: Glutamine signaling specifically activates c-Myc and Mcl-1 to facilitate cancer cell proliferation and survival
    Article Snippet: .. Whole length human FBW7, QARS, c-MYC, MCL-1, NOTCH1, CCNE1, and JUN were amplified from HCT116 +/+ cDNA and cloned into the Xho I and EcoR I restriction sites of the pcDNA3.1-Flag/HA/Myc vector using CloneExpress multiS One Step Cloning Kit (#C113-02, Vazyme, Nanjing, China). .. Site-directed mutagenesis of FBW7 was performed with the Mut Express MultiS Fast Mutagenesis kit (#C215-01, Vazyme).

    Article Title: Glutamine signaling specifically activates c-Myc and Mcl-1 to Facilitate cancer cell proliferation and survival.
    Article Snippet: .. Whole length human FBW7, QARS, c-MYC, MCL-1, NOTCH1, CCNE1 and JUN were amplified from HCT116 +/+ cDNA and cloned into the Xho I and EcoR I restriction sites of the pcDNA3.1-Flag/HA/Myc vector using CloneExpress multiS One Step Cloning Kit (#C11302, Vazyme, Nanjing, China). .. Site-directed mutagenesis of FBW7 was performed with the Mut Express MultiS Fast Mutagenesis kit (#C215-01, Vazyme).

    Article Title: Tryptophanylation of insulin receptor by WARS attenuates insulin signaling
    Article Snippet: .. Plasmids construction and transfection Whole length human WARS, IR, and SIRT1 were amplified HEK293T cDNA and cloned into Xho I and EcoR I sites of the pcDNA3.1-Flag/HA vector using CloneExpress MultiS One Step Cloning Kit (Vazyme, #C113-02, CHN). .. The mutants were generated by site-directed mutagenesis using the Mut Express MultiS Fast Mutagenesis Kit (Vazyme, #C215-01, CHN) according to the manufacturer’s instructions.

    Article Title: Tryptophanylation of insulin receptor by WARS attenuates insulin signaling
    Article Snippet: .. Whole length human WARS, IR, and SIRT1 were amplified HEK293T cDNA and cloned into Xho I and EcoR I sites of the pcDNA3.1-Flag/HA vector using CloneExpress MultiS One Step Cloning Kit (Vazyme, #C113-02, CHN). .. The mutants were generated by site-directed mutagenesis using the Mut Express MultiS Fast Mutagenesis Kit (Vazyme, #C215-01, CHN) according to the manufacturer’s instructions.

    Transfection:

    Article Title: Tryptophanylation of insulin receptor by WARS attenuates insulin signaling
    Article Snippet: .. Plasmids construction and transfection Whole length human WARS, IR, and SIRT1 were amplified HEK293T cDNA and cloned into Xho I and EcoR I sites of the pcDNA3.1-Flag/HA vector using CloneExpress MultiS One Step Cloning Kit (Vazyme, #C113-02, CHN). .. The mutants were generated by site-directed mutagenesis using the Mut Express MultiS Fast Mutagenesis Kit (Vazyme, #C215-01, CHN) according to the manufacturer’s instructions.



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